Established in 1925 by robert dawley, the original sprague dawley rat colony was obtained by harlan in 1980 through the acquisition of spraguedawley, inc. Report of partial findings from the national toxicology. In the first study, groups of 40 male and 40 female spraguedawley rats were treated with 1, 2, 4, or 6 to 8 gkg b. Pdf reference values for young normal spraguedawley rats. The sprague dawley rat aka sd, is an albino outbred rat with an elongated head and a tail that is longer than its body. Male spraguedawley rats 250280g were supplied by the laboratory animal service center, the chinese university of hong kong cuhk. Genetic characterization of outbred sprague dawley rats. Dawley from a hybrid hooded male and a female wistar rat. Journal of hazardous materials 306 2016 95104 99 the injection in the pleuralperitoneal space of the rats, the calculated. Metabolic differences between sprague dawley and wistar han. A control group of 60 rats per sex was fed the same diet without apm. The mean life span of normal female spraguedawley rats in this laboratory was 760 21 days, with individuals ranging in life span from 193 to 1100 days.
Nih received stock from sprague dawley, inc in 1945. No toxic effects were observed till the end of the study. Sprague dawley rat an overview sciencedirect topics. Female sprague dawley rats show impaired spatial memory in. Untreated female rats were approximately 10 weeks at start of cohabitation. Research paper charles river sprague dawley rats lack. Spontaneous tumors in spraguedawley rats and swiss mice1.
Sexspecific reference intervals of hematologic and. Partial findings from the ntp carcinogenesis studies of cell. The sprague dawley breed of rat, like those used in the current psychology 381 class, is used widely throughout medical and psychological research. Sprague dawley sd rat has a wide array of historical research use including in the field of cardiology. Our research goal was to calculate a dose of dmba at each age in which 50% of the rats developed carcinoma in situ cis within three months ic50 in this strain of sprague dawley female rats. Rederived into an isolator foundation colony in 1997.
A total of 219 neoplasms were observed in 165 60 males and 105 females of the 360 rats examined. Evaluation of biological and environmental variability franck chanut 1,carie kimbrough 2,rick hailey 2,brian berridge 2,angela hughesearle 3,rhiannon davies 1, kimberly roland 3,alan stokes 2,alessandro casartelli 4,malcolm york 1,holly jordan 2,federica crivellente 4, patrizia. Evaluation of immunomodulatory parameters in female sprague. Ninetyday nephrotoxicity evaluation of 3mcpd 1monooleate. Spontaneous cardiomyopathy in young spraguedawley rats. History of the sprague dawley rat erikacarlys weblog. Comparative analysis of growth characteristics of sprague. Request pdf metabolic differences between sprague dawley and wistar han male rats while sprague dawley sd rats are often used for nonclinical safety testing, wistar han wh rats are also. Arterial blood from 63 male 315500 g and 60 female 210290 g healthy spraguedawley rats was analyzed for 16 hematological and 22 clinical. The treated animals were kept under observation for 2 days, for mortality and general behaviour. Studies in hypertensive rats support this hypothesis. A number of rat species are used in research, contract research organizations and in the pharmaceutical industry including the wistar, sprague dawley and fischer 344.
Fatty acid esters of 3monochloropropane 1,2diol 3mcpd esters are processinginduced food toxicants, with the kidney as their major target organ. Spraguedawley rat definition of spraguedawley rat by. Reference intervals as presently defined for these analytes in sprague dawley rats have not used. This suggests that cucumis sativus extract possesses antidiabetic effect on alloxaninduced diabetic spraguedawley rat and therefore can be used as an alternative therapy in treating diabetes mellitus. The sprague dawley sd is an outbred rat, first generated by robert s. Mean, standard deviation, median interquartile range as well as minimum and maximum values for the. Bp was measured at baseline and four weekly for 24 weeks. Spraguedawley rats maintained on diet containing 265 ppm cobalt for 98 days showed degenerative changes in the testes considered to be secondary to hypoxia mollenhauer et al.
Squamous cell papilloma is differentiated from the squamous cell carcinoma by its exophytic growth and the absence of invasion of the corneal stroma by atypical squamous cells. Cd sprague dawley igs rat charles river laboratories. Mean, standard deviation, median interquartile range. Background hematologic and biochemical analytes of sprague dawley rats are commonly used to determine effects that were induced by treatment and to evaluate organ dysfunction in toxicological safety assessments, but reference intervals have not been well established for these analytes. Rift valley fever virus rvfv infections in pregnant livestock cause high rates of fetal demise. The adult body weight is 250300g for females, and 450520g for males. Charles river provides laboratory animal models, including the sprague dawley rat, for use in basic research and nonclinical drug discovery and. Radiation rats 22018 telephone press conference audio. More than 85% of all rats were alive at the end of the experi ment at day 540, as shown in table 1. Metabolic differences between sprague dawley and wistar. Taconic received stock from the nih animal genetic resource in 1970. Report of final results regarding brain and heart tumors. Haematological profile shows that inbred sprague dawley rats. The occurrences of two tumor types in male harlan sprague dawley rats exposed to rfr, malignant.
The aim of the present study was to explore if dim white 7. Charles river sprague dawley rats lack early agedependent. In 1991, 8 colonies were selected to form the igs foundation colony. Outbred sprague dawley rats are among the most commonly used rats for. The sprague dawley rat is an outbred multipurpose breed of albino rat used extensively in medical research. Sprague dawley and wistar are the main rat strains. Sprague dawley rats maintained on diet containing 265 ppm cobalt for 98 days showed degenerative changes in the testes considered to be secondary to hypoxia mollenhauer et al. To address how rvfv infection during pregnancy causes detrimental effects on the fetus, we developed a pregnant rodent model of rvfv infection. Dmba, mammary carcinogenesis, charles river sprague dawley rats. We will breed up to 3 founders to wildtype rats of matching strain background and genotype their offspring to obtain f1 rats bearing the transgene. Our data show that spatial memory significantly improved within 5 daily.
Probiotic amelioration of azotemia in 56th nephrectomized. Feeding regimen is also believed to have a role, as sprague dawley sd and wistar rats on a restricted. Administration of 2acetylaminofluorene 2aaf to rats increased mdrlb expression in fischer, wistar and sprague dawley rat livers. An acute toxicity test was performed in sd rats of both genders and the lethal dose ld50 of per oral gardenia yellow was estimated to be more than 15. Administration of 2acetylaminofluorene 2aaf to rats increased mdrlb expression in fischer, wistar and spraguedawley rat livers. Antidiabetic effect of zingiber officinale on sprague dawley. In fischer 344 rats, squamous cell carcino mas have exhibited papillary growth patterns. It is the most widely used rat model for biomedical research. None of the deaths at less than 1 year were associated with neoplasia. Male and female spraguedawley rats were exposed from prenatal life until natural death to a 1. It should be noted that perinatal lethality was induced in pregnant spraguedawley rats by prenatal exposure to des at 45. The sprague dawley outbred model was developed by sprague dawley, inc. The rat is the most commonly used rodent species for toxicology studies.
The rats are maintained as an outbred closed colony. Carcinogenicity studies in spraguedawley sd rats show that pfoa induces the tumor triad pattern liver adenomas, leydig cell tumors, and pancreatic acinar cell tumors similar to several peroxisome proliferatoractivated receptor alpha ppar. Sprague dawley rat definition of sprague dawley rat by. Reference intervals as presently defined for these analytes in spraguedawley rats have not used. Treatment on the herbomineral formulation in female sprague dawley rats. However, you may choose to have us breed the founders further to obtain f1 rats. Tumor incidence in normal spraguedawley female rats cancer. The jackson laboratory, charles river laboratories, taconic farms, and harlan laboratories are the main suppliers. I am a model organism used by duke university to research the impacts of chemical exposure on behavior, memory, and cognitive. Rift valley fever virus induces fetal demise in sprague. Like other rat breeds used in research, the sprague dawley makes for a good general model for the study of human toxicology, reproduction, pharmacology, and behavioral research. Wistar and sd rats were assigned for 2 experimental groups for 17 weeks. Tumor incidence in normal spraguedawley female rats.
Comparing ntp and ri studies of spraguedawley rats. Spraguedawley sd and long evans strains are available for transgenic rat production. Investigation of cytochrome p450 1a2 and 3a inhibitory properties of danshen tincture. The occurrences of two tumor types in male harlan sprague dawley rats exposed to rfr, malignant gliomas in the. The objective of this study was to evaluate the toxic characteristics of rauvolfia tetraphylla leaf extract in sprague dawley rats after treatment for minimum 7 days by oral route. Hypoglycemic activity of cucumis sativus extract on. Male spraguedawley rats 90120g body weight bred and maintained at the biomedical research unit, university of kwazulunatal was used in this study. Light intensity and wavelength strongly influence mood and cognition in humans and rodent animal models. In 1991, 8 colonies were selected to form the igs foundation colony using the charles river international genetic standard system. A summary and overview of the mice and rats used in biomedical research, based on a survey of formal publications. Ntp draft conclusions, transcript, youtube of audio 212018 niehs press release dna damage genotoxicity findings report of final results regarding brain and heart tumors in spraguedawley rats exposed from prenatal life until natural death to mobile phone. Male sprague dawley rats 250280g were supplied by the laboratory animal service center, the chinese university of hong kong cuhk. Cd igs rat model information sheet charles river laboratories. Dawley during the 1920s by breeding wistar rats to hybrids of laboratory derived and wild stocks.
Cdsdigs br rats were obtained from charles river laboratories raleigh, nc. Evaluation of the chronic toxicity and carcinogenicity of. The treatment started at 4 weeks of age and lasted for a period of 104 weeks. Scientific poster maternal and embryofetal historical background control data in the hsd. The present study was to test the hypothesis that selected bacteria instilled into the gastrointestinal tract could help in converting nitrogenous wastes accumulated due to renal insufficiency into nontoxic compounds. The purpose of this communication is to report partial findings from a series of radiofrequency. For the in vivo tests on sprague dawley rats, single 25mg injection of each mineral.
The rats average about 5 grams of food per day per 100 grams of body weight and about 1012 milliliters of water per 100 grams of body weight. Regulation of mdrlb gene expression in fischer, wistar and. Haematological profile shows that inbred sprague dawley. The spraguedawley sd is an outbred rat, first generated by robert s. In the subchronic study, gardenia yellow was orally. To investigate the basis of this difference we further examined the regulation of the mdrlb gene in hepatocytes isolated from fischer, sprague. Regardless of the health standard selected, taconic. Evaluation of immunomodulatory parameters in female. One part was denoted as the control without any biofield energy treatment, while the other part was defined as the biofield energy treated sample, which received the biofield energy.
A 1972 study compared neoplasms in sprague dawley rats from six different commercial suppliers and found highly significant differences in the incidences of endocrine and mammary tumors. Herein we describe a prospective, blinded, placebocontrolled pilot study, using 56 th nephrectomized sprague dawley. Jul 15, 2015 however, a direct comparison between wistar and sprague dawley sd rat as models of highfat hf dietinduced obesity has not been adequately evaluated so far. Reduced sperm motility andor increased abnormal sperm were noted in mice, but not in rats, exposed to 3 mgm 3 or higher in week inhalation studies with. Spraguedawley and wistar rats are both established models of sucroseinduced ir and hypertg pagliassotti et al. The average litter size of the sprague dawley rat is 10.
It has been shown that hypertensive rats developed vascular diseases predominantly located in the cardiac microvasculature yamori and okomoto 1976. For the first time, this study treated sprague dawley sd rats with 3mcpd 1monooleate at 10 and 100 mgkg bwday and 1monostearate at 15 and 150 mgkg bwday for 90 days and examined for their potential semilongterm nephrotoxicity and the. Animals from low, mid and high dose groups received leaf extract at 250, 500 and mgkgday, respectively. In the first study, groups of 40 male and 40 female sprague dawley rats were treated with 1, 2, 4, or 6 to 8 gkg b. Male rats were approximately 6 weeks of age at the start of dosing and were assigned to groups based on a weightbalanced random allocation scheme. Research paper charles river sprague dawley rats lack early. Pubertal development and reproductive functions of crl. Mound laboratory is operated by monsanto chemical company for the united states atomic energy commission under contract number at331gen53. About sprague dawley rats lauren and heathers blog. I am a model organism used by duke university to research the impacts of chemical exposure on behavior, memory, and cognitive ability. Comparative analysis of growth characteristics of sprague dawley.
In 1950, charles river laboratories crl acquired a breeding stock and developed a new line with better microbial status by caesarian derivation 22. Antidiabetic effect of zingiber officinale on sprague. Sprague dawley rat detailed specifications janvier labs. The study was conducted according to oecd guideline 407. Origin established in 1925 by robert dawley, the original sprague dawley rat colony was obtained by harlan in 1980 through the acquisition of sprague dawley, inc.
Partial findings from the ntp carcinogenesis studies of. Pdf comparative analysis of growth characteristics of sprague. There were even significant variations in the incidences of adrenal medulla tumors among rats from the same source raised in different laboratories. Try out the sas sprague dawley rat with our animal model evaluation program. Assessment of female and male fertility in spraguedawley. These extracts were given to three groups n 6 of rats at concentrations, 1500 and 2000 mgkg body weight. Background hematologic and biochemical analytes of spraguedawley rats are commonly used to determine effects that were induced by treatment and to evaluate organ dysfunction in toxicological safety assessments, but reference intervals have not been well established for these analytes. Sprague dawley rats obtained from different sources. The aim of the study was to evaluate the immunomodulatory potential of a biofield energy healing the trivedi effect treatment on the herbomineral formulation in female sprague dawley rats. Evaluation of immunomodulatory parameters in female sprague dawley rats after oral administration of the biofield energy healing treated herbomineral formulation, american journal of bioscience. Dec 22, 2015 the sprague dawley sd is an outbred rat, first generated by robert s.
This study was conducted to evaluate the acute and subchronic toxicity of gardenia yellow, a natural colorant widely used in china and other asian countries. The animals had free access to standard rat chow meadows feeds, pietermaritzburg, south africa and water, with a 12hour light12hhour dark cycle. Dawley rats were randomly divided into three groups n10, including the control, oa and oa with treadmill exercise oae groups. These rats typically have increased tail to body length ratio compared with wistar rats. National toxicology program ntp has carried out extensive rodent toxicology and carcinogenesis studies of radiofrequency radiation rfr at frequencies and modulations used in the u. This report presents partial findings from these studies. To investigate the basis of this difference we further examined the regulation of the mdrlb gene in hepatocytes isolated from fischer, spraguedawley and wistar rats. Effect of consumption of fresh and heated virgin coconut oil on the blood pressure and inflammatory biomarkers.
The rats were refreshed with nih genetic resource stock in 1998. Almost all laboratory and domestic pet rats belong to a single species, the norway rat rattus norvegicus. Thirty male spraguedawley rats were divided into five groups and were fed with the following diet for 24 weeks. Pdf genetic background in animal models is an intrinsic research variable in. Sprague dawley rats were first bred by the sprague dawley farm in madison, wisconsin in 1925 through multiple crosses with a singlehooded male and six albino female rats. Mccomb dj, kovacs k, beri j, zak f 1984 pituitary adenoma in old spraguedawley rats. An automated haematology analyzer was used to determine haematological indices of whole blood from individual in 20 each of male and female inbred sprague dawley rats.
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